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im gonna give give 10 whopping points to whoever can translate this into easy to understand language:
Assemblies were tested against honeybee data sets (expressed sequence tags (ESTs), complementary DNAs, microsatellite markers and sequenced bacterial artificial chromosome (BAC) clones) for quality and completeness. The qualityy of version3 and version4 was tested by alignment of full-length cDNAs (55 total). All alignments showed correct order and orientation of exons.
Although a limited set of cDNAs, it nevertheless seems that misassembly of genic regions is rare. In version4, 99% of 2,032 markers and 98% of 3,136 ESTs were represented, providing evidence for completeness.
When aligned to BACs that had been independently sequenced, 23 out of 27 BACs showed >94% coverage, whereas four were >80% but were complex owing to repeated sequences. The estimated clonable genome size is thus taken as 231 Mb/98% = 236 Mb.
It sounds like they were comparing artificially produced DNA sequences to honeybee sequences.
It merely states the level of permutation is very low in all the data sets tested (the BACs, ESTs, microsatellites and cDNAs)
In version 4, 99% of the DNA markers were where they were supposed to be. In version 3 98% of the markers were where they were supposed to be...
In total, 23/27 samples had >94% of the DNA sequence correct, while the other 4/27 had >80% of the DNA correct.
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